2.1 Follow standard ELISA procedures.
block and wash
Dispense analyte. Incubate and wash microplate.
Add HRP conjugate. Incubate and wash microplate.
Per insert instruction, mix equal parts of chemiluminescent
substrate solution A
and solution B.
Store in an amber
Add mixed chemiluminescent substrate solution to each well.
Mix liquid in wells if required.
Place the microplate on a luminometer and measure photocount
units) between 1-5