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                                       ►    Reagent - Chemiluminescent Substrate

 

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Substrate List

   ►   Product Information

Luminol base reagents
Immediate light generation-high signal is produced immediately at room temperature
Femtogram to Nanogram sensitivity
High signal-to-noise ratios-minimal background
Easy mix-two-component reagents
Stable for a minimum of 12 months at 2-8C

   ►   Example of Assay

Microplate Procedure for Detection of Horseradish Peroxidase

Materials
 

1.1     96-well microplate, black or white opaque

1.2    HRP at 0.4 25 ng/ml

1.3    Buffer for HRP dilution

   
Procedure
 

1.1     Per insert instruction, mix equal parts of chemiluminescent substrate solution A and solution B.  Store in an amber bottle.

1.2     Dispense 10 l of HRP solution into the microwell.

1.3     Add 100ul of mixed chemiluminescent substrate solution.

1.4     Place the microplate on a luminometer and measure photocount (relative light units) between 1- 5 minutes.

ELISA Microplate Procedure

Materials
 

1.1     96-well microplate, black or white opaque

1.2     Diluted capture antibody

1.3     Wash buffer

1.4     Block buffer

1.5     Analyte

1.6     HRP-Antibody conjugate

   
Procedure
 

2.1    Follow standard ELISA procedures.  Coat, incubate, block and wash microplate.

2.2    Dispense analyte.  Incubate and wash microplate.

2.3    Add HRP conjugate.  Incubate and wash microplate.

2.4    Per insert instruction, mix equal parts of chemiluminescent substrate solution A and solution B. Store in an amber bottle.

2.5    Add mixed chemiluminescent substrate solution to each well.

2.6    Mix liquid in wells if required.

2.7    Place the microplate on a luminometer and measure photocount (relative light units) between 1-5 minutes

 

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   ►   Experiment Data

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   ►   Chemiluminescent Substrate List

Part No.

Description

44-C001

HRP LuminMax Plus (Horseradish Peroxidase) Luminescent Substrate
 

2-Component 2x25ml

LuminMax Plus HRP Chemiluminescent Substrate, Solution A 25ml

LuminMax Plus HRP Chemiluminescent Substrate, Solution B 25ml

44-C003 HRP LuminMax Plus Chemiluminescent Substrate

 

2-Component 2x50ml

LuminMax Plus HRP Chemiluminescent Substrate, Solution A 50ml

LuminMax Plus HRP Chemiluminescent Substrate, Solution B 50ml

44-C011

HRP LuminMax Super Chemiluminescent Substrate

 

2-Component 2x25ml

LuminMax Super HRP Chemiluminescent Substrate, Solution A 25ml

LuminMax Super HRP Chemiluminescent Substrate, Solution B 25ml

44-C013

HRP LuminMax Super Chemiluminescent Substrate

 

2-Component 2x50ml

LuminMax Super HRP Chemiluminescent Substrate, Solution A 50ml

LuminMax Super HRP Chemiluminescent Substrate, Solution B 50ml

   ►   To Order

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  • Call Sales Department at 562-801-2088 extension 229, Monday through Friday, 9:00 a.m. to 5:30 p.m. PST

  • Fax your order to us at 562-801-2089

  • E-mail us at sales@maxwellsensors.com

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